Roche’s global presence 22 Research and development sites in Pharmaceuticals and Diagnostics worldwide 26 Manufacturing sites in Pharmaceuticals and Diagnostics worldwide Key figures CHF 50, million Group sales +4%** 27 million patients treated with one of our top 25 medicines 29 Roche medicines on the WHO List of Essential Medicines. Alternatively, the proteins were digested sequentially by trypsin (as described above) and Asp-N protease (20 ng, 6 h; Roche, Mannheim, Germany) as well as using the sequence Asp-N and trypsin, always in the same cleavage buffer The digestions were stopped by addition of 5 µL of 5% trifluoroacetic acid in MeCN. Asp-N endoproteinase was purchased from Roche Applied Science (Mannheim, Germany), Lys-C endopeptidase by Wako Chemicals USA (Richmond, VA) and mass spectrometry-grade trypsin by Promega (Madison, WI).

Asp n roche pdf

Alternatively, the proteins were digested sequentially by trypsin (as described above) and Asp-N protease (20 ng, 6 h; Roche, Mannheim, Germany) as well as using the sequence Asp-N and trypsin, always in the same cleavage buffer The digestions were stopped by addition of 5 µL of 5% trifluoroacetic acid in MeCN. which cleaves peptide bonds N-terminally at small hydrophilic amino acids (1). A mutant of this strain produces the Endoproteinase Asp-N, which is isolated as a highly purified and specific protease. Sequence of Endoproteinase Asp-N Partial sequences of Endoproteinase Asp-N are described (4, 5). Quality Control. Asp-N endoproteinase was purchased from Roche Applied Science (Mannheim, Germany), Lys-C endopeptidase by Wako Chemicals USA (Richmond, VA) and mass spectrometry-grade trypsin by Promega (Madison, WI). Asp-N activity is optimal in the pH range of – This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. Roche’s global presence 22 Research and development sites in Pharmaceuticals and Diagnostics worldwide 26 Manufacturing sites in Pharmaceuticals and Diagnostics worldwide Key figures CHF 50, million Group sales +4%** 27 million patients treated with one of our top 25 medicines 29 Roche medicines on the WHO List of Essential Medicines.N-terminal side of aspartic and cysteic acid residues (1,2). Biological Usage Note: Asp-N, Sequencing Grade, is lyophilized in the presence of Tris-HCl. Roche products will be available to purchase through the award- winning tecnohispanos.com Roche .. Endoproteinase Asp-N Sequencing grd, 2ug . Endoproteinase Asp-N, Sequencing Grade. from a mutant of Pseudomonas fragi, lyophilizate. For further processing only. expand | collapse. +-. asp n roche pdf free. Quote. Postby Just» Sat Mar 2, am. Looking for asp n roche pdf free. Will be grateful for any help! Top. Endoproteinase Asp-N Sequencing Grade from Pseudomonas fragi; find Roche- ENDOARGS-RO MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Pseudomonas fragi · Bulletin (PDF).

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