Roche’s global presence 22 Research and development sites in Pharmaceuticals and Diagnostics worldwide 26 Manufacturing sites in Pharmaceuticals and Diagnostics worldwide Key figures CHF 50, million Group sales +4%** 27 million patients treated with one of our top 25 medicines 29 Roche medicines on the WHO List of Essential Medicines. Alternatively, the proteins were digested sequentially by trypsin (as described above) and Asp-N protease (20 ng, 6 h; Roche, Mannheim, Germany) as well as using the sequence Asp-N and trypsin, always in the same cleavage buffer The digestions were stopped by addition of 5 µL of 5% trifluoroacetic acid in MeCN. Asp-N endoproteinase was purchased from Roche Applied Science (Mannheim, Germany), Lys-C endopeptidase by Wako Chemicals USA (Richmond, VA) and mass spectrometry-grade trypsin by Promega (Madison, WI).
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